|
| Status |
Public on Apr 30, 2023 |
| Title |
Synchronized L4/young adult pgph-2 oe animals grown on NGM - 4 |
| Sample type |
SRA |
| |
|
| Source name |
Whole extract
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: Whole extract
developmental stage: L4/young adult
genotype: pgph-2 oe
treatment: NGM
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol-chloroform followed by purification with Quiagen RNA purification kit.
Libraries were prepared using NEBNext Ultra II RNA Library Prep by Illumina. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
pgp_4
|
| Data processing |
Raw data of fastq format were processed through in-house perl scripts. The clean data were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. All the downstream analyses were based on the clean data with high quality.
gene_count.xls generated using featureCounts 1.5.0-p3
Assembly: WBcel235
Supplementary files format and content: gene_count.xls of raw gene counts
|
| |
|
| Submission date |
Apr 03, 2023 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Marcas Agostinelli |
| E-mail(s) |
marcus.agostinelli@gmail.com
|
| Phone |
8144047650
|
| Organization name |
Novogene
|
| Department |
Bioinformatics
|
| Street address |
2690 W Avenida Azahar
|
| City |
Tucson |
| State/province |
Arizona |
| ZIP/Postal code |
85745 |
| Country |
USA |
| |
|
| Platform ID |
GPL26672 |
| Series (1) |
| GSE228784 |
RNA seq to determine differential gene expression in pgph-2 oe C.elegans animals and the subset of HLH-30-dependent genes on normal growth and excess glucose conditions. |
|
| Relations |
| BioSample |
SAMN34046433 |
| SRA |
SRX19847800 |
