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Sample GSM7136496 Query DataSets for GSM7136496
Status Public on Apr 30, 2023
Title Synchronized L4/young adult pgph-2 oe animals grown on 2% glucose - 4
Sample type SRA
 
Source name Whole extract
Organism Caenorhabditis elegans
Characteristics tissue: Whole extract
developmental stage: L4/young adult
genotype: pgph-2 oe
treatment: 2% glucose
Extracted molecule polyA RNA
Extraction protocol Trizol-chloroform followed by purification with Quiagen RNA purification kit.
Libraries were prepared using NEBNext Ultra II RNA Library Prep by Illumina. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description pgp_gluc_4
Data processing Raw data of fastq format were processed through in-house perl scripts. The clean data were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. All the downstream analyses were based on the clean data with high quality.
gene_count.xls generated using featureCounts 1.5.0-p3
Assembly: WBcel235
Supplementary files format and content: gene_count.xls of raw gene counts
 
Submission date Apr 03, 2023
Last update date Apr 30, 2023
Contact name Marcas Agostinelli
E-mail(s) marcus.agostinelli@gmail.com
Phone 8144047650
Organization name Novogene
Department Bioinformatics
Street address 2690 W Avenida Azahar
City Tucson
State/province Arizona
ZIP/Postal code 85745
Country USA
 
Platform ID GPL26672
Series (1)
GSE228784 RNA seq to determine differential gene expression in pgph-2 oe C.elegans animals and the subset of HLH-30-dependent genes on normal growth and excess glucose conditions.
Relations
BioSample SAMN34046429
SRA SRX19847804

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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