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Sample GSM7146674 Query DataSets for GSM7146674
Status Public on Aug 08, 2023
Title Mouse Lung tumor_KrasLA1;DicerS2D_rep3
Sample type RNA
 
Source name primary lung tumor
Organism Mus musculus
Characteristics gender: female
age in weeks: 36w
strain: C57B6J
tissue: lung tumor
genotype: KrasLA1/+;Dicer1S2D/+
Treatment protocol No treatment. This are genetically modified mouse models that carry oncogenic Kras G12D (Kras LA1/+); and phosphomimetic Dicer1 wherein serines at positions 1712 and 1836 were replaced to aspartic acid in a oncogenic Kras G12D background (KrasLA1/+;Dicer1S2D/+)
Growth protocol 30 to 37 weeks old mice from both genotypes were euthanized following IACUC protocol
Extracted molecule total RNA
Extraction protocol Lungs were removed from mouse. Then tumors were identified and collected under the dissecting microscope. 50ug of tumors were homogenized in 1 mL of Trizol (Ambion, #15596018). Total RNA was purified following miRNeasy Mini Kit (Qiagen, #217004). 100ng of total RNA was used for nCounter Nanostring miRNA (NS_M_MIR_V1.5) assay.
Label miRNA reported and capture probes
Label protocol nCounter Nanostring mouse miRNA (NS_M_MIR_V1.5) assay with capture probes which are complementary to miRNAs sequence
 
Hybridization protocol Hybridization was performed following nCounter Nanostring miRNA (NS_M_MIR_V1.5) protocol in where miRNA reporter codeset, hybridization buffer and miRNA capture probe set was added to each sample.
Scan protocol Nanostring nCounter instrunent
Description miRNAs were assayed using Ncounter Nanostring mouse miRNAs v1.5
Data processing Results were analyzed in nCounter Analysis Software v4.0. Statistical analysis was performed using two-tailed t-test and Benjamin-Yekutieli for false discovery rate.
 
Submission date Apr 04, 2023
Last update date Aug 08, 2023
Contact name Raisa Reyes-Castro
E-mail(s) RAReyes2@mdanderson.org
Organization name UT Health MD Anderson Cancer Center
Department Genetics
Lab Swathi Arur
Street address 6767 Bertner Ave
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
 
Platform ID GPL33314
Series (2)
GSE228966 Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas [Nanostring]
GSE228968 Phosphorylated nuclear DICER1 promotes open chromatin state and gastric cell fate in lung adenocarcinomas

Data table header descriptions
ID_REF
VALUE Normalized counts

Data table
ID_REF VALUE
4 255.75
5 7801.48
6 1682.97
7 2674.55
8 339.2
9 238.41
10 766.17
11 573.27
12 39.15
13 60.69
14 39.15
15 46.6
16 39.15
17 39.15
18 39.15
19 39.15
20 39.15
21 166.89
22 39.15
23 39.15

Total number of rows: 626

Table truncated, full table size 6 Kbytes.




Supplementary file Size Download File type/resource
GSM7146674_20210413_31020932336_390_05.RCC.gz 5.2 Kb (ftp)(http) RCC
Processed data included within Sample table
Processed data are available on Series record

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