|
| Status |
Public on Jun 30, 2023 |
| Title |
h926_het_RA |
| Sample type |
SRA |
| |
|
| Source name |
right atria
|
| Organism |
Mus musculus |
| Characteristics |
tissue: right atria
cell type: Cardiac
genotype: Zfhx3 Het
treatment: NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from murine cardiac tissue using the Direct-zol RNA Miniprep kit (Zymo Research, R2051).
RNA-seq libraries were generated with an mRNA enrichment protocol (KAPA mRNA HyperPrep, 08098115702). All RNA-seq libraries were sequenced on an Illumina NovaSeq.
|
| |
|
| Library strategy |
ssRNA-seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
rep3_het_RA
|
| Data processing |
Sequenced reads were aligned to the mouse genome (mm10) using STAR (2.7.9a) (PMID: 23104886). Differential expression analysis was performed with DESeq2 (1.30.1) (PMID: 25516281). Pathway enrichment analysis was carried out with Metascape (PMID: 30944313).
Assembly: mm10
Supplementary files format and content: Gene counts table constructed of raw output from STAR.
|
| |
|
| Submission date |
Apr 12, 2023 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Matthew C Hill |
| E-mail(s) |
mhill@broadinstitute.org
|
| Organization name |
Broad Institute of MIT and Harvard
|
| Street address |
75 Aimes Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE229525 |
Loss of the Atrial Fibrillation-related gene, Zfhx3, Results in Atrial Dilation and Arrhythmias by Disruption of Genes Essential for Cardiovascular Homeostasis |
|
| Relations |
| SRA |
SRX19946374 |
| BioSample |
SAMN34157117 |
