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Sample GSM7181536 Query DataSets for GSM7181536
Status Public on Apr 18, 2024
Title Group A Normal
Sample type RNA
 
Source name Serum collected from the patient
Organism Homo sapiens
Characteristics tissue: Pooled serum from 12 patients with normal confirmed by histology
Extracted molecule total RNA
Extraction protocol RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
Label Cy5
Label protocol Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
 
Hybridization protocol Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
Scan protocol 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
Description A total of 2 micro gram of extracted total RNA from the pooled samples in each disease category was prepared and 250 ng of this was labelled with a 3D-Gene miRNA labelling kit (Toray) followed by hybridizing onto 3D-Gene Human miRNA Oligo chips (Toray).
1 nomal
Data processing The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
 
Submission date Apr 18, 2023
Last update date Apr 18, 2024
Contact name Satoshi Kondo
Organization name Toray Industries,Inc.
Street address 10-1 tebiro 6-chome
City Kamakura
State/province Kanagawa
ZIP/Postal code 248-8555
Country Japan
 
Platform ID GPL21263
Series (1)
GSE229935 microRNA profile in the serum of the cervical neoplasia

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
MIMAT0000062 111.037106
MIMAT0000063 133.869417
MIMAT0000064 117.551372
MIMAT0000065 92.321162
MIMAT0000066 23.769879
MIMAT0000067 38.232625
MIMAT0000068 125.91245
MIMAT0000069 641.07333
MIMAT0000070 183.947871
MIMAT0000071 28.413764
MIMAT0000072 23.058073
MIMAT0000073 45.623328
MIMAT0000074 186.485022
MIMAT0000075 133.120992
MIMAT0000076 66.179009
MIMAT0000077 200.267345
MIMAT0000078 325.140874
MIMAT0000079 19.768312
MIMAT0000080 170.941509
MIMAT0000081 184.353158

Total number of rows: 2565

Table truncated, full table size 55 Kbytes.




Supplementary file Size Download File type/resource
GSM7181536_SH57664_Cell1.txt.gz 62.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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