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Sample GSM7181540 Query DataSets for GSM7181540
Status Public on Apr 18, 2024
Title Group B Normal
Sample type RNA
 
Source name Serum collected from the patient
Organism Homo sapiens
Characteristics tissue: Pooled serum from 20 patients with normal confirmed by histology
Extracted molecule total RNA
Extraction protocol RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
Label Cy5
Label protocol Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
 
Hybridization protocol Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
Scan protocol 3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
Description A total of 2 micro gram of extracted total RNA from the pooled samples in each disease category was prepared and 250 ng of this was labelled with a 3D-Gene miRNA labelling kit (Toray) followed by hybridizing onto 3D-Gene Human miRNA Oligo chips (Toray).
Normal
Data processing The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
 
Submission date Apr 18, 2023
Last update date Apr 18, 2024
Contact name Satoshi Kondo
Organization name Toray Industries,Inc.
Street address 10-1 tebiro 6-chome
City Kamakura
State/province Kanagawa
ZIP/Postal code 248-8555
Country Japan
 
Platform ID GPL21263
Series (1)
GSE229935 microRNA profile in the serum of the cervical neoplasia

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
MIMAT0000062 19.587665
MIMAT0000063 28.339025
MIMAT0000064 21.10727
MIMAT0000065 20.542259
MIMAT0000066 5.314429
MIMAT0000067
MIMAT0000068 17.40644
MIMAT0000069 97.156208
MIMAT0000070 12.003525
MIMAT0000071
MIMAT0000072
MIMAT0000073
MIMAT0000074 11.095146
MIMAT0000075 8.537034
MIMAT0000076
MIMAT0000077 16.505442
MIMAT0000078 45.147909
MIMAT0000079
MIMAT0000080 216.701663
MIMAT0000081 18.301899

Total number of rows: 2565

Table truncated, full table size 49 Kbytes.




Supplementary file Size Download File type/resource
GSM7181540_SH81N25_Cell2.txt.gz 62.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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