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Sample GSM7182104 Query DataSets for GSM7182104
Status Public on Nov 12, 2023
Title Spleen plasmablasts, biol rep 2
Sample type SRA
 
Source name Spleen
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Spleen
cell type: Plasmablasts
Sex: female
Extracted molecule polyA RNA
Extraction protocol RNeasy Plus Mini kit (Qiagen).
TruSeq RNA Sample preparation kit (Illumina)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description Sp.PB_2
Data processing Sequencing was undertaken on an Illumina NextSeq 500 to produce 86bp single-end reads.
Read alignment was performed with the align function of the Rsubread package.
Genewise read counts were obtained with the featureCounts function of the Rsubread package and Rsubread's inbuilt mm10 annotation.
Assembly: mm10
Supplementary files format and content: Tab-delimited text files with columns for Entrez Gene ID, gene length (number of bases in union of all exons), and the number of reads. The supplementary file GenewiseCounts.txt.gz gives read counts for all samples in one file.
 
Submission date Apr 18, 2023
Last update date Nov 12, 2023
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL19057
Series (1)
GSE229970 Unraveling the diversity and functions of tissue-resident plasma cells (RNA-seq of mouse antibody-secreting cells)
Relations
BioSample SAMN34234107
SRA SRX20001184

Supplementary file Size Download File type/resource
GSM7182104_Sp.PB_2.txt.gz 169.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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