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Sample GSM7188015 Query DataSets for GSM7188015
Status Public on Oct 03, 2023
Title NonTreated_GFPneg, KT14
Sample type SRA
 
Source name NonTreated_GFPneg
Organism Mus musculus
Characteristics cell type: Tumor infiltrating lymphocytes
sorted cell type: CD8+ T cells
genotype: GFP negative
treatment: No aPDL1 treatment
Extracted molecule total RNA
Extraction protocol Total RNA extracted from cells using RNAeasy (Qiagen) kit as per manufacturers instructions
Quant-seq 3′ mRNA-seq Library Prep Kit for Illumina (Lexogen) as per manufacturer’s instructions.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Illumina Casava1.8.2 software used for basecalling.
The RNA sequencing reads were trimmed using cutadapt(v1.7) to remove poor quality ends.
Read data was then aligned using Hisat2 to the mouse reference genome (mm10)
Read counting was performed with featureCounts from the subread package (v1.6.4)
Assembly: mm10
Supplementary files format and content: count file generated by featureCounts
 
Submission date Apr 20, 2023
Last update date Oct 03, 2023
Contact name Kevin C sek
E-mail(s) kevin.sek@petermac.org
Phone 0432960331
Organization name Peter MacCallum Cancer Centre
Department Cancer Immunology
Lab Beavis Lab
Street address 305 Grattan Street
City Melbourne
State/province VIC
ZIP/Postal code 3000
Country Australia
 
Platform ID GPL19057
Series (1)
GSE230135 A2AR-eGFP reporter mouse enables elucidation of A2AR expression dynamics during anti-tumor immune responses
Relations
BioSample SAMN34264554
SRA SRX20021997

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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