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Sample GSM7188108 Query DataSets for GSM7188108
Status Public on Dec 01, 2023
Title GXpoly_10
Sample type RNA
 
Source name Colon mucosa of a rat fed a diet containging GXpoly, replicate 10
Organism Rattus norvegicus
Characteristics strain: Wistar
Sex: female
date: 2021-01-25
treatment: extract rich in polyphenol and glucoronoxylan
sample type: GXpoly
tissue type: mucosa of colon mid-segment
cage: 12
slide: 1
slide position: 1_3
rin: 8.1
group: 3
260/280 ratio: 2.12
Treatment protocol The four-weeks-old female and male Wistar (RccHan: WIST) rats purchased from Envigo Netherlands (Horst, The Netherlands) were fed pelleted, modified AIN-93G diets (Envigo Teklad Diets, Madison WI, The United States) containing either 10% (w/w) of cellulose (control group), 10% of polyphenol-and GX-rich extract (GXpoly), or 10% of highly purified GX (pureGX) after 12 days of acclimatization.
Growth protocol The animal study protocol was approved by the Finnish National Animal Experiment Board (Eläinkoelautakunta, ELLA; permit code: ESAVI/12806/2019), and the research was carried out according to ARRIVE guidelines.
Extracted molecule total RNA
Extraction protocol For RNA extraction, the mucosa of colon mid-segment (2cm) was collected using a microscope slide and stored in RNALater for 24 hours at +4°C, after which it was transferred to -20°C. Total RNA was extracted with TRIzol Plus RNA Purification Kit (12183555, Invitrogen) according to manufactures instructions. Samples with RIN values > 8 were used for the analysis.
Label Cy3
Label protocol Samples (200 ng) were amplified using the T7 RNA polymerase method. cRNAs were labelled with Cy3 or Cy5 following the manufacturer's protocol (Agilent).
 
Hybridization protocol Labeled cRNA samples were hybridised onto the Agilent 2-colour 8x60k arrays according to the manufacturer's recommendations and hybridized for 17 hours at 65°C. After hybridization, slides were washed following the manufacturer's instructions (Agilent).
Scan protocol Slides were scanned with Agilent microarray scanner G2505C and data were extracted using the Agilent Feature Extraction software (V12.0.2.2.)
Description US11263921_257403611528_S01_GE2_1200_Jun14_1_3.txt
Gene expression from the colon mucosa of a female rat fed a diet containing polyphenol-and GX-rich extract (10% w/w)
PAm37
Data processing Raw intensity values were obtained from Agilent Feature Extraction software and quality checked according to Agilent standard procedures. The median foreground intensities were imported into R software and data were log2 transformed and normalized with quantile normalization. This experiment was performed as a two-colour experiment, but data were analyzed as single-channel arrays. Raw data files are available as a tar archive on the series record.
 
Submission date Apr 20, 2023
Last update date Dec 01, 2023
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL22145
Series (1)
GSE230139 Diet supplemented with polyphenol- and glucuronoxylan-rich fiber extract regulates colonic barrier function and cell proliferation in healthy rats

Data table header descriptions
ID_REF
VALUE Log2 transformed and quantile normalized values

Data table
ID_REF VALUE
1 13.70471904
2 5.506000896
3 5.506000896
4 5.746735067
5 5.631630611
6 6.064324474
7 5.807616402
8 9.59123761
9 7.218295814
10 5.608431991
11 6.094844514
12 13.41962839
13 8.843864646
14 6.41366418
15 6.491647522
16 5.900347192
17 6.108303903
18 6.554140934
19 5.936592311
20 6.019004881

Total number of rows: 62976

Table truncated, full table size 1091 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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