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Sample GSM718999 Query DataSets for GSM718999
Status Public on May 03, 2011
Title Biceps femoris muscle_AQM_Treatment_2
Sample type RNA
 
Source name biceps femoris muscle, treated with non-depolarizing neuromuscular blocking agents(NMBA),Corticosteroids(CS) and sepsis
Organism Sus scrofa
Characteristics breed: Eurasian wild boar
gender: female
disease state: acute quadriplegic myopathy (AQM)
treatment: non-depolarizing neuromuscular blocking agents (NMBA),Corticosteroids(CS) and sepsis
time: 5 days
tissue: biceps femoris muscle
Treatment protocol Five female domestic piglets (Sus scrofa, average body weight 26.9 kg) were used in this study. Five subjects were included for masseter muscle and same four subjects were included for the biceps femoris muscle. Piglets were immobilized by anesthesia and mechanically ventilated via a tracheotomy for a period of five days. During this study period, the animals were sedated using isoflurane inhalation (Abbott Laboratories, Chicago, Il, USA, 0.8 – 1.3% end-tidal concentration) supplemented by intravenous bolus doses of morphine and ketamine as needed. Core body temperature (blood) was maintained in the range of 38.5 – 40°C by a servo controlled heating pad. The animals received intravenous crystalloid fluid (Ringeracetat) to maintain stable blood pressure and urinary output and a glucose infusion (Rehydrex, Fresenius Kabi, Stockholm, Sweden, 25 mg glucose /mL) in the range of 0.5 – 1.5 mg/kg/minute to decrease the effects of catabolism. Each animal received prophylactic streptomycin 750 mg/d and bensylpenicillin 600 mg/d (Streptocillin Vet, Boeringer-Ingelheim, Hellerup, Denmark). Arterial blood gas analysis as well as electrolytes and blood glucose levels were monitored regularly and kept in the normal range throughout the study period. A neuromuscular blocking agent (NMBA) was administered as a continuous infusion of pancuronium bromide 0.1mg/kg/h (Pavolun; Organon, Boxtel, The Netherlands) for 5days while a corticosteroid (CS) was given as bolus doses of betamethasone 0.1 mg/kg (Betapred; GSK, Slona, Sweden) twice daily for 5days.Endotoxemia was induced by a continuous infusion of Escherichia coli endotoxin, serotype O26:B6 (Sigma Labkemi, Stockholm, Sweden) at 36 µg/kg/h for 1h.
Growth protocol n/a
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the standard Affymetrix protocol including Trizol extraction and Rneasy column cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description RLPA-Day5_N_CS_S-2PC.CEL
RLPA-Day5_N_CS_S-2PC
Data processing The data were normalized using RMA.
 
Submission date May 03, 2011
Last update date May 03, 2011
Contact name sudhakar reddy aare
Organization name uppsala university
Department neuroscience
Lab clinical neurophysiology
Street address Ing 85, 3 tr, Akademiska sjukhuset
City uppsala
ZIP/Postal code 751 85
Country Sweden
 
Platform ID GPL3533
Series (1)
GSE24239 Mechanisms underlying the sparing of masticatory muscle function relative to biceps femoris muscle in an experimental critical illness model

Data table header descriptions
ID_REF
VALUE RMA-normalized log2 signal

Data table
ID_REF VALUE
Ssc.10953.1.A1_at 9.472038
Ssc.1952.1.S1_at 11.38187
Ssc.24998.1.S1_a_at 10.83103
Ssc.2655.1.A1_at 9.674689
Ssc.3669.1.A1_at 9.150927
Ssc.26039.1.S1_at 10.27289
Ssc.25098.1.S1_at 7.618598
Ssc.4844.1.S1_at 6.529752
Ssc.13371.1.S1_at 11.48638
Ssc.16336.1.S1_at 10.23242
Ssc.16843.1.S1_at 10.60941
Ssc.21839.1.S1_at 10.62697
Ssc.20598.1.A1_at 10.80869
Ssc.908.3.S1_at 9.358211
Ssc.18187.2.S1_at 7.731657
Ssc.4123.1.S1_at 12.55014
Ssc.16843.2.S1_at 9.287728
Ssc.6930.1.A1_at 6.770443
Ssc.2850.2.S1_a_at 8.051476
Ssc.1655.1.A1_at 9.209923

Total number of rows: 24123

Table truncated, full table size 628 Kbytes.




Supplementary file Size Download File type/resource
GSM718999.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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