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Sample GSM7233873 Query DataSets for GSM7233873
Status Public on Apr 30, 2023
Title amniotic fluid P167-750knsp
Sample type genomic
 
Source name amniotic fluid P167
Organism Homo sapiens
Characteristics cell type: amniotic fluid
indications of invasive prenatal diagnosis: isolated increased NT
offspring gender: male
Treatment protocol None
Growth protocol Cell were grown according to standard conditions.
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from uncultured amniotic fluid, fetal cord blood using a QIAGEN kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
Label Biotin
Label protocol As per manufacturer (Affymetrix)
 
Hybridization protocol DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
Data processing The array image was acquired using Affymetrix GeneChip® Operating Software (ChAS 4.0). Copy number values for individual SNPs were extracted and converted from CEL files into signal intensities using GTYPE 4.1 and Affymetrix Copy Number Analysis Tool (CNAT 4.0.1) softwares. Genomic Smoothing analysis was performed by using the smoothing window of 0 Mb, and inferred copy number states were derived from a Hidden Markov Model (HMM) based algorithm implemented in CNAT 4.0.1. Circular Binary Segmentation (Ohlsen et al., 2004) was applied using DNAcopy package for R Bioconductor on raw data.
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
 
Submission date Apr 27, 2023
Last update date Apr 30, 2023
Contact name su linjuan
E-mail(s) candy_2005518@126.com
Phone 15280422904
Organization name Fujian Provincial Maternity and Children's Hospital,
Street address daoshanlu
City fuzhou
ZIP/Postal code 350001
Country China
 
Platform ID GPL18637
Series (1)
GSE230763 Can non-invasive prenatal screening based on cell-free fetal DNA be utilized to assess chromosome abnormalities in fetuses with increased Nuchal Translucency?

Supplementary file Size Download File type/resource
GSM7233873_20170607_155928_006_P167.cy750K.cychp.gz 24.8 Mb (ftp)(http) CYCHP
Processed data provided as supplementary file

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