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Sample GSM724446 Query DataSets for GSM724446
Status Public on May 20, 2011
Title TRAMP_serum_advancedDiseased_rep3
Sample type RNA
Source name Serum from TRAMP mice with advanced disease
Organism Mus musculus
Characteristics tissue: serum
strain: C57BL/6 TRAMP x FVB
genotype/variation: TRAMP
age: mixed
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted twice with phenol:chloroform and precipitated using ethanol.
Label biotin
Label protocol Approximately 800ng of RNA was labelled using the FlashTag HSR Biotin RNA Labelling Kit (Genisphere), according to the manufacturer's instructions.
Hybridization protocol cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Arrays and then washed and stained with a Steptavidin-PE solution prior to imaging.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description miRNA data from TRAMP serum
Data processing Data were background corrected and statistically analysed using Partek Genomics Suite (Partek Inc.) using the subset of mouse probes on the array. Raw data was processed using RMA background correction and Partek’s own GC content correction with mean probe summarization.
Submission date May 16, 2011
Last update date May 20, 2011
Contact name Luke Selth
Organization name Flinders University
Department College of Medicine and Public Health
Street address Flinders Drive
City Bedford Park
State/province SA
ZIP/Postal code 5042
Country Australia
Platform ID GPL8786
Series (1)
GSE29314 Identification of serum microRNAs associated with prostate cancer in a mouse model of disease

Data table header descriptions
VALUE RMA signal intensity

Data table
sme-miR-10_st -0.0427985
fru-miR-210_st 2.1283
ENSG00000202231_x_st 0.30347
hsa-miR-423-5p_st 2.30602
cfa-miR-138a_st 1.08519
vvi-miR535e_st 0.0900356
U95_st 0.669765
ptc-miR474a_st 0.473162
mdo-miR-181b_st 0.0173954
ame-miR-31a_st 0.289804
sme-miR-71c_st 1.3149
mml-miR-138_st 0.248855
ENSG00000207002_x_st 1.84537
ame-miR-315_st 0.387735
cfa-miR-196b_st 0.912957
xtr-miR-202-star_st 1.08383
mmu-miR-1195_st 3.40403
U43_st 0.68247
ath-miR830-star_st 0.523792
AFFX-BkGr23-GC10_st 0.394074

Total number of rows: 7815

Table truncated, full table size 189 Kbytes.

Supplementary file Size Download File type/resource
GSM724446.CEL.gz 121.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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