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Sample GSM724448 Query DataSets for GSM724448
Status Public on May 20, 2011
Title WT_serum_rep2
Sample type RNA
Source name Serum from wild-type littermate control mice
Organism Mus musculus
Characteristics tissue: serum
strain: C57BL/6 x FVB
genotype/variation: wild type
age: mixed
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted twice with phenol:chloroform and precipitated using ethanol.
Label biotin
Label protocol Approximately 800ng of RNA was labelled using the FlashTag HSR Biotin RNA Labelling Kit (Genisphere), according to the manufacturer's instructions.
Hybridization protocol cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Arrays and then washed and stained with a Steptavidin-PE solution prior to imaging.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description miRNA data from WT serum
Data processing Data were background corrected and statistically analysed using Partek Genomics Suite (Partek Inc.) using the subset of mouse probes on the array. Raw data was processed using RMA background correction and Partek’s own GC content correction with mean probe summarization.
Submission date May 16, 2011
Last update date May 20, 2011
Contact name Luke Selth
Organization name Flinders University
Department College of Medicine and Public Health
Street address Flinders Drive
City Bedford Park
State/province SA
ZIP/Postal code 5042
Country Australia
Platform ID GPL8786
Series (1)
GSE29314 Identification of serum microRNAs associated with prostate cancer in a mouse model of disease

Data table header descriptions
VALUE RMA signal intensity

Data table
sme-miR-10_st 0.526226
fru-miR-210_st 0.505206
ENSG00000202231_x_st 0.609538
hsa-miR-423-5p_st 0.39489
cfa-miR-138a_st -0.0943554
vvi-miR535e_st 0.418254
U95_st 0.616866
ptc-miR474a_st -0.241039
mdo-miR-181b_st 1.02729
ame-miR-31a_st 0.91504
sme-miR-71c_st 0.152766
mml-miR-138_st -0.141009
ENSG00000207002_x_st 0.348084
ame-miR-315_st 0.0510757
cfa-miR-196b_st -0.0424228
xtr-miR-202-star_st 0.0712214
mmu-miR-1195_st 0.153174
U43_st -0.0324574
ath-miR830-star_st 0.0999893
AFFX-BkGr23-GC10_st 0.672985

Total number of rows: 7815

Table truncated, full table size 190 Kbytes.

Supplementary file Size Download File type/resource
GSM724448.CEL.gz 136.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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