|
| Status |
Public on May 19, 2011 |
| Title |
108 |
| Sample type |
RNA |
| |
|
| Source name |
Mesothelioma tumor, epi
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: malignant pleural mesothelioma (MPM)
subtype: epi
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy plus mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the GeneChip Human U133A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Scanner.
|
| Description |
Gene expression data from MPM tumors.
|
| Data processing |
The data is normalized through the RMA method with default parameters using Bioconductor.
|
| |
|
| Submission date |
May 17, 2011 |
| Last update date |
May 19, 2011 |
| Contact name |
Yupu Liang |
| E-mail(s) |
liangy@rockefeller.edu
|
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE29211 |
The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma |
| GSE29354 |
Mesothelioma tumor gene expression profiles |
|
