|
| Status |
Public on May 19, 2011 |
| Title |
Parental HARA |
| Sample type |
RNA |
| |
|
| Source name |
squamous cell lung cancer cell line (HARA)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HARA
tissue: squamous cell lung cancer
|
| Growth protocol |
HARA and HARA-B4 were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% FBS and 50 units/ml of penicillin and streptomycin in humidified atmosphere under 5% CO2 at 37˚C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from HARA and HARA-B4 cells using the Qiagen RNeasy kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from human squamous cell lung cancer cell line, HARA
|
| Data processing |
The data were analyzed with Affymetrix GeneChip Command Console and normalized with Affymetrix Expression Console.
|
| |
|
| Submission date |
May 18, 2011 |
| Last update date |
May 19, 2011 |
| Contact name |
Soichi Takiguchi |
| E-mail(s) |
sctakigu@nk-cc.go.jp
|
| Organization name |
National Kyushu Cancer Center
|
| Street address |
3-1-1 Notame, Minami-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
811-1395 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29367 |
Expression data from human squamous cell lung cancer line HARA and highly bone metastatic subline HARA-B4. |
|
