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Sample GSM728875 Query DataSets for GSM728875
Status Public on Jan 11, 2012
Title Blood AEC012
Sample type other
 
Source name Cellular fraction of peripheral human blood
Organism Homo sapiens
Characteristics sample: Asbestos-exposed control
age: 76
gender: Male
smoking: No
Extracted molecule other
Extraction protocol MicroRNA isolation of blood cells seperated from plasma by centrifugation was perfomed using the RiboPure-Blood Kit (Applied Biosystems) according to the alternate protocol: Isolation of Small RNAs.
Label Cy5
Label protocol Labeling of microRNA was performed using 5´-phosphate-cytidyl-cytidyl-Cy5-3´ (Eurogentec) according to the miRNA Microarray System Protocol (Agilent Technologies).
 
Hybridization protocol Hybridization was performed using the microarray hybridization station HS 400 Pro (Tecan, Männedorf, Switzerland) according to Liu et al. (2008) MicroRNA expression profiling using microarrays. Nat Protoc 3: 563-578.
Scan protocol Microarrays scanning at 635 nm for Cy5 and signal definitions were performed according to Weber et al. (2008) Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. J Appl Microbiol 105: 951-962.
Data processing Generated data were analyzed using GeneSpring GX 11.0 (Agilent Technologies). Data transformation measurements less than 0.01 were set to 0.01 and normalization per microarray was performed using the 50th percentile.
Only human microRNAs were analyzed during the study
 
Submission date May 23, 2011
Last update date Jan 11, 2012
Contact name Daniel Gilbert Weber
Organization name IPA
Department Molekulare Medizin
Street address Bürkle-de-la-Camp Platz 1
City Bochum
ZIP/Postal code 44789
Country Germany
 
Platform ID GPL13618
Series (1)
GSE29707 Analysis of deregulated microRNAs in malignant mesothelioma

Data table header descriptions
ID_REF
VALUE Normalized values

Data table
ID_REF VALUE
hsa_let_7a -2.6392794
hsa_let_7b 1.1084614
hsa_let_7c -1.798284
hsa_let_7d -2.0570827
hsa_let_7e -0.44115257
hsa_let_7f -1.2515175
hsa_let_7g -2.1192806
hsa_let_7i -0.8140347
hsa_miR_1 -0.48748535
hsa_miR_100 -0.3876782
hsa_miR_101 -1.7320418
hsa_miR_103 -1.4751444
hsa_miR_105 -0.6868694
hsa_miR_106a -0.82394695
hsa_miR_106b -1.8160026
hsa_miR_107 -0.52526355
hsa_miR_10a -0.8229636
hsa_miR_10b -2.1771219
hsa_miR_122a -0.10581392
hsa_miR_124a -1.857118

Total number of rows: 328

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM728875.csv.gz 67.8 Kb (ftp)(http) CSV
Processed data included within Sample table

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