|
| Status |
Public on Jan 11, 2012 |
| Title |
Blood AEC017 |
| Sample type |
other |
| |
|
| Source name |
Cellular fraction of peripheral human blood
|
| Organism |
Homo sapiens |
| Characteristics |
sample: Asbestos-exposed control
age: 56
gender: Male
smoking: Ex
|
| Extracted molecule |
other |
| Extraction protocol |
MicroRNA isolation of blood cells seperated from plasma by centrifugation was perfomed using the RiboPure-Blood Kit (Applied Biosystems) according to the alternate protocol: Isolation of Small RNAs.
|
| Label |
Cy5
|
| Label protocol |
Labeling of microRNA was performed using 5´-phosphate-cytidyl-cytidyl-Cy5-3´ (Eurogentec) according to the miRNA Microarray System Protocol (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was performed using the microarray hybridization station HS 400 Pro (Tecan, Männedorf, Switzerland) according to Liu et al. (2008) MicroRNA expression profiling using microarrays. Nat Protoc 3: 563-578.
|
| Scan protocol |
Microarrays scanning at 635 nm for Cy5 and signal definitions were performed according to Weber et al. (2008) Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. J Appl Microbiol 105: 951-962.
|
| Data processing |
Generated data were analyzed using GeneSpring GX 11.0 (Agilent Technologies). Data transformation measurements less than 0.01 were set to 0.01 and normalization per microarray was performed using the 50th percentile.
Only human microRNAs were analyzed during the study
|
| |
|
| Submission date |
May 23, 2011 |
| Last update date |
Jan 11, 2012 |
| Contact name |
Daniel Gilbert Weber |
| Organization name |
IPA
|
| Department |
Molekulare Medizin
|
| Street address |
Bürkle-de-la-Camp Platz 1
|
| City |
Bochum |
| ZIP/Postal code |
44789 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13618 |
| Series (1) |
| GSE29707 |
Analysis of deregulated microRNAs in malignant mesothelioma |
|
