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Sample GSM728884 Query DataSets for GSM728884
Status Public on Jan 11, 2012
Title Blood AEC022
Sample type other
 
Source name Cellular fraction of peripheral human blood
Organism Homo sapiens
Characteristics sample: Asbestos-exposed control
age: 62
gender: Male
smoking: Yes
Extracted molecule other
Extraction protocol MicroRNA isolation of blood cells seperated from plasma by centrifugation was perfomed using the RiboPure-Blood Kit (Applied Biosystems) according to the alternate protocol: Isolation of Small RNAs.
Label Cy5
Label protocol Labeling of microRNA was performed using 5´-phosphate-cytidyl-cytidyl-Cy5-3´ (Eurogentec) according to the miRNA Microarray System Protocol (Agilent Technologies).
 
Hybridization protocol Hybridization was performed using the microarray hybridization station HS 400 Pro (Tecan, Männedorf, Switzerland) according to Liu et al. (2008) MicroRNA expression profiling using microarrays. Nat Protoc 3: 563-578.
Scan protocol Microarrays scanning at 635 nm for Cy5 and signal definitions were performed according to Weber et al. (2008) Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. J Appl Microbiol 105: 951-962.
Data processing Generated data were analyzed using GeneSpring GX 11.0 (Agilent Technologies). Data transformation measurements less than 0.01 were set to 0.01 and normalization per microarray was performed using the 50th percentile.
Only human microRNAs were analyzed during the study
 
Submission date May 23, 2011
Last update date Jan 11, 2012
Contact name Daniel Gilbert Weber
Organization name IPA
Department Molekulare Medizin
Street address Bürkle-de-la-Camp Platz 1
City Bochum
ZIP/Postal code 44789
Country Germany
 
Platform ID GPL13618
Series (1)
GSE29707 Analysis of deregulated microRNAs in malignant mesothelioma

Data table header descriptions
ID_REF
VALUE Normalized values

Data table
ID_REF VALUE
hsa_let_7a 3.7781906
hsa_let_7b 0.2143178
hsa_let_7c -0.72663593
hsa_let_7d -9.673765
hsa_let_7e -0.10087824
hsa_let_7f -0.6656172
hsa_let_7g -15.275748
hsa_let_7i 3.0456903
hsa_miR_1 -0.64417547
hsa_miR_100 -0.79989725
hsa_miR_101 0.04766321
hsa_miR_103 3.986443
hsa_miR_105 1.2080967
hsa_miR_106a -7.45195
hsa_miR_106b 0.17712045
hsa_miR_107 -1.3004386
hsa_miR_10a 0.9708985
hsa_miR_10b 1.1841309
hsa_miR_122a -3.3927903
hsa_miR_124a 4.120756

Total number of rows: 328

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM728884.csv.gz 78.4 Kb (ftp)(http) CSV
Processed data included within Sample table

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