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Sample GSM728886 Query DataSets for GSM728886
Status Public on Jan 11, 2012
Title Blood MMP001
Sample type other
 
Source name Cellular fraction of peripheral human blood
Organism Homo sapiens
Characteristics sample: Malignant mesothelioma patient
age: 84
gender: Male
smoking: Ex
histological subtype: Biphasic
Extracted molecule other
Extraction protocol MicroRNA isolation of blood cells seperated from plasma by centrifugation was perfomed using the RiboPure-Blood Kit (Applied Biosystems) according to the alternate protocol: Isolation of Small RNAs.
Label Cy5
Label protocol Labeling of microRNA was performed using 5´-phosphate-cytidyl-cytidyl-Cy5-3´ (Eurogentec) according to the miRNA Microarray System Protocol (Agilent Technologies).
 
Hybridization protocol Hybridization was performed using the microarray hybridization station HS 400 Pro (Tecan, Männedorf, Switzerland) according to Liu et al. (2008) MicroRNA expression profiling using microarrays. Nat Protoc 3: 563-578.
Scan protocol Microarrays scanning at 635 nm for Cy5 and signal definitions were performed according to Weber et al. (2008) Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria. J Appl Microbiol 105: 951-962.
Data processing Generated data were analyzed using GeneSpring GX 11.0 (Agilent Technologies). Data transformation measurements less than 0.01 were set to 0.01 and normalization per microarray was performed using the 50th percentile.
Only human microRNAs were analyzed during the study
 
Submission date May 23, 2011
Last update date Jan 11, 2012
Contact name Daniel Gilbert Weber
Organization name IPA
Department Molekulare Medizin
Street address Bürkle-de-la-Camp Platz 1
City Bochum
ZIP/Postal code 44789
Country Germany
 
Platform ID GPL13618
Series (1)
GSE29707 Analysis of deregulated microRNAs in malignant mesothelioma

Data table header descriptions
ID_REF
VALUE Normalized values

Data table
ID_REF VALUE
hsa_let_7a 0.55824375
hsa_let_7b 0.85990715
hsa_let_7c 1.1744533
hsa_let_7d 1.2656655
hsa_let_7e 2.2840605
hsa_let_7f 2.2170885
hsa_let_7g 0.6603372
hsa_let_7i 0.609339
hsa_miR_1 -0.056351125
hsa_miR_100 -0.85927445
hsa_miR_101 -1.0559068
hsa_miR_103 0.19974041
hsa_miR_105 0.3871734
hsa_miR_106a 0.1044817
hsa_miR_106b -0.8225615
hsa_miR_107 1.1846836
hsa_miR_10a -5.262709
hsa_miR_10b -2.2329843
hsa_miR_122a 0.809825
hsa_miR_124a -0.6552638

Total number of rows: 328

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM728886.csv.gz 69.8 Kb (ftp)(http) CSV
Processed data included within Sample table

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