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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 20, 2024 |
| Title |
scRNA-Seq of skin, IMQ |
| Sample type |
SRA |
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| Source name |
Skin
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| Organism |
Mus musculus |
| Characteristics |
tissue: Skin
strain: C57BL/6
age: post natal 7 weeks
treatment: imiquimod
condition: imiquimod-induced psoriasis
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| Extracted molecule |
total RNA |
| Extraction protocol |
The mice project from dorsal skin
RNA: 3' transcriptome sequencing, cDNA fragmentation, end filling, adding A, connection connector, Index PCR
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
The IMQ group was treated with imiquimod
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| Data processing |
Sample demultiplexing, barcode processing, and single cell 3’ gene counting were performed by the Cell Ranger pipelines (v3.1.0,10X Genomics).
Assembly: mm10 (GRCm38)
Supplementary files format and content: barcodes.tsv; features.tsv; matrix.mtx
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| Submission date |
May 04, 2023 |
| Last update date |
Apr 20, 2024 |
| Contact name |
Jiao Qu |
| E-mail(s) |
171850015@smail.nju.edu.cn
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| Organization name |
Nanjing University
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| Street address |
Nanjing University, 163 Xianlin Avenue, Nanjing 210023, China
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| City |
Nanjing |
| ZIP/Postal code |
210023 |
| Country |
China |
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| Platform ID |
GPL17021 |
| Series (1) |
| GSE231728 |
Celastrol targets LRP1 to inhibit fibroblast-macrophage crosstalk and ameliorates psoriasis progression |
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| Relations |
| BioSample |
SAMN34590321 |
| SRA |
SRX20228503 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7299704_IMQ_barcodes.tsv.gz |
34.6 Kb |
(ftp)(http) |
TSV |
| GSM7299704_IMQ_genes.tsv.gz |
244.7 Kb |
(ftp)(http) |
TSV |
| GSM7299704_IMQ_matrix.mtx.gz |
71.8 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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