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Sample GSM730030 Query DataSets for GSM730030
Status Public on Dec 08, 2011
Title e17.5 fat tissue
Sample type RNA
Source name developing adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: developing adipose tissue
developmental stage: e17.5
Treatment protocol RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
Extracted molecule total RNA
Extraction protocol RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Developing mouse adipose tissue pooled from at least 3 embryos
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
Submission date May 24, 2011
Last update date Dec 08, 2011
Contact name kivanc birsoy
Organization name Rockefeller University
Street address 1230 Rockefeller University
City new york
ZIP/Postal code 10065
Country USA
Platform ID GPL6885
Series (1)
GSE29502 Gene expression profile of developing adipose tissue in vivo

Data table header descriptions
VALUE quantile normalized

Data table
ILMN_1250052 7.13025585
ILMN_3122480 6.776111043
ILMN_2599935 8.28953935
ILMN_2675543 6.880987474
ILMN_2686883 6.88891028
ILMN_2751818 6.857463873
ILMN_2728634 7.057787443
ILMN_3040515 6.817675652
ILMN_2711608 6.873709538
ILMN_1232875 6.932004247
ILMN_1258507 6.879166571
ILMN_2746142 6.79294401
ILMN_1252690 6.937753469
ILMN_2655499 6.692483124
ILMN_1252870 6.993136047
ILMN_1248179 6.805929286
ILMN_2649955 7.287447938
ILMN_2628708 7.654267983
ILMN_3024781 6.811343381
ILMN_2705628 7.41809411

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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