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Sample GSM730031 Query DataSets for GSM730031
Status Public on Dec 08, 2011
Title e18.5 fat tissue
Sample type RNA
 
Source name developing adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: developing adipose tissue
developmental stage: e18.5
Treatment protocol RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
Extracted molecule total RNA
Extraction protocol RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Developing mouse adipose tissue pooled from at least 3 embryos
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date May 24, 2011
Last update date Dec 08, 2011
Contact name kivanc birsoy
E-mail(s) kbirsoy@wi.mit.edu
Organization name Rockefeller University
Street address 1230 Rockefeller University
City new york
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE29502 Gene expression profile of developing adipose tissue in vivo

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1250052 7.207192203
ILMN_3122480 6.813503477
ILMN_2599935 8.298011781
ILMN_2675543 6.862726542
ILMN_2686883 6.861430578
ILMN_2751818 6.861430578
ILMN_2728634 7.100876662
ILMN_3040515 6.8508739
ILMN_2711608 6.863764209
ILMN_1232875 6.848501111
ILMN_1258507 6.959912815
ILMN_2746142 6.733284885
ILMN_1252690 6.940400766
ILMN_2655499 6.840135653
ILMN_1252870 7.053690519
ILMN_1248179 6.878134006
ILMN_2649955 7.522184291
ILMN_2628708 7.301116124
ILMN_3024781 6.837895093
ILMN_2705628 8.760385334

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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