NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM730032 Query DataSets for GSM730032
Status Public on Dec 08, 2011
Title P0 fat tissue
Sample type RNA
 
Source name developing adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: developing adipose tissue
developmental stage: P0
Treatment protocol RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
Extracted molecule total RNA
Extraction protocol RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Developing mouse adipose tissue pooled from at least 3 embryos
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date May 24, 2011
Last update date Dec 08, 2011
Contact name kivanc birsoy
E-mail(s) kbirsoy@wi.mit.edu
Organization name Rockefeller University
Street address 1230 Rockefeller University
City new york
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE29502 Gene expression profile of developing adipose tissue in vivo

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1250052 7.174120387
ILMN_3122480 6.761175985
ILMN_2599935 8.04847716
ILMN_2675543 6.864059461
ILMN_2686883 6.960401993
ILMN_2751818 6.86510194
ILMN_2728634 7.065665768
ILMN_3040515 6.897077737
ILMN_2711608 6.948194745
ILMN_1232875 6.876066713
ILMN_1258507 6.894532115
ILMN_2746142 6.835502505
ILMN_1252690 6.895326398
ILMN_2655499 6.853003151
ILMN_1252870 6.932155321
ILMN_1248179 6.828566537
ILMN_2649955 7.253080007
ILMN_2628708 6.990855308
ILMN_3024781 6.848501111
ILMN_2705628 8.047477546

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap