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Sample GSM730034 Query DataSets for GSM730034
Status Public on Dec 08, 2011
Title P5 fat tissue
Sample type RNA
Source name developing adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: developing adipose tissue
developmental stage: P5
Treatment protocol RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
Extracted molecule total RNA
Extraction protocol RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Developing mouse adipose tissue pooled from at least 3 embryos
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
Submission date May 24, 2011
Last update date Dec 08, 2011
Contact name kivanc birsoy
Organization name Rockefeller University
Street address 1230 Rockefeller University
City new york
ZIP/Postal code 10065
Country USA
Platform ID GPL6885
Series (1)
GSE29502 Gene expression profile of developing adipose tissue in vivo

Data table header descriptions
VALUE quantile normalized

Data table
ILMN_1250052 7.207192203
ILMN_3122480 6.866143685
ILMN_2599935 8.106792339
ILMN_2675543 6.939016468
ILMN_2686883 6.899642183
ILMN_2751818 6.806704465
ILMN_2728634 7.048280736
ILMN_3040515 6.847446785
ILMN_2711608 6.843606712
ILMN_1232875 6.848501111
ILMN_1258507 6.938025044
ILMN_2746142 6.755251307
ILMN_1252690 6.962357037
ILMN_2655499 6.821187379
ILMN_1252870 6.977315189
ILMN_1248179 6.847446785
ILMN_2649955 7.219984533
ILMN_2628708 7.078405412
ILMN_3024781 6.836400167
ILMN_2705628 6.888468377

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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