|
| Status |
Public on May 26, 2011 |
| Title |
CD34+CD45RA+Lin- HPCs transduced with empty vector cultured onto 2 µg of Delta1ext-IgG |
| Sample type |
RNA |
| |
|
| Source name |
CD34+CD45RA-Lin- HPCs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: umbilical cord blood
cell type: hematopoietic progenitors
genotype/variation: transduced with empty vector
treatment group: Notch-activated (2 µg)
|
| Treatment protocol |
CD34+CD45RA-Lin-HPCs were exposed to A2M or control lentiviral vectors, and sorted again based on GFP expression. After 72-hr culture with 0, 2 or 5 µg of Delta1-ext-IgG, cells were harvested and processed for RNA extraction
|
| Growth protocol |
Cell were cultured under serum free condtion in the presence of SCF, FL and TPO with 0, 2 or 5µg of plastic-immobilized Delta1ext-IgG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted using the RNeasy Micro Kit
|
| Label |
biotin
|
| Label protocol |
RNA samples were subjected to linear amplification using the MessageAMP Ambion amplification kit
|
| |
|
| Hybridization protocol |
cRNAs were then hybridized to Affymetrix HG-U133 plus 2.0 GeneChip arrays
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner 2500A
|
| Description |
BCE_03
Gene expression data from Notch-activated (2 µg) cells transduced with empty vector
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
May 25, 2011 |
| Last update date |
May 26, 2011 |
| Contact name |
Marc Delord |
| E-mail(s) |
mdelord@gmail.com
|
| Organization name |
Centre hospitalier de Versailles
|
| Department |
Recherche clinique
|
| Street address |
Hopital Saint-Louis, 1 avenue Claude Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29524 |
Expression data from Notch-activated CD34+CD45RA-Lin- hematopoietic progenitor cells (HPCs) transduced with nuclear-trapped AF1q/MLLT11 (A2M) |
|
