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Sample GSM7316136 Query DataSets for GSM7316136
Status Public on Jun 09, 2023
Title X5b
Sample type SRA
Source name K562 cell line
Organism Homo sapiens
Characteristics cell line: K562 cell line
cell type: lymphoblast cells
genotype: Cas9FCPF+sgRNA
treatment: JQ1
time: 1h
Treatment protocol Transfected with Cas9FCPF with/without sgRNA
1 µM JQ1 or 1 µM JQ1-FCPF
Growth protocol K562 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, Quality control and sequencing were performed by Azenta
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing RNA seq was performed by Azenta
Submission date May 10, 2023
Last update date Jun 09, 2023
Contact name Xinlai Cheng
Organization name Goethe university Frankfurt
Department Pharmaceutical Chemistry
Street address Max-von-Laue-Strasse 15. R. 3.652
City Frankfurt
ZIP/Postal code D-60438
Country Germany
Platform ID GPL16791
Series (1)
GSE232132 Chem-CRISPR/dCas9 -a chemically induced platform for epigenome editing
BioSample SAMN35019520
SRA SRX20275795

Supplementary file Size Download File type/resource
GSM7316136_5b.counts.txt.gz 4.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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