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Sample GSM7316141 Query DataSets for GSM7316141
Status Public on Jun 09, 2023
Title X9a
Sample type SRA
 
Source name K562 cell line
Organism Homo sapiens
Characteristics cell line: K562 cell line
cell type: lymphoblast cells
genotype: Cas9FCPF+sgRNA
treatment: JQ1-FCPF
time: 1h
Treatment protocol Transfected with Cas9FCPF with/without sgRNA
1 µM JQ1 or 1 µM JQ1-FCPF
Growth protocol K562 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, Quality control and sequencing were performed by Azenta
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing RNA seq was performed by Azenta
 
Submission date May 10, 2023
Last update date Jun 09, 2023
Contact name Xinlai Cheng
E-mail(s) Cheng@pharmchem.uni-frankfurt.de
Organization name Goethe university Frankfurt
Department Pharmaceutical Chemistry
Street address Max-von-Laue-Strasse 15. R. 3.652
City Frankfurt
ZIP/Postal code D-60438
Country Germany
 
Platform ID GPL16791
Series (1)
GSE232132 Chem-CRISPR/dCas9 -a chemically induced platform for epigenome editing
Relations
BioSample SAMN35019515
SRA SRX20275800

Supplementary file Size Download File type/resource
GSM7316141_9a.counts.txt.gz 4.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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