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Sample GSM7349921 Query DataSets for GSM7349921
Status Public on Sep 27, 2023
Title intact spleen, biological rep 3
Sample type RNA
Source name spleen RNA
Organism Mus musculus
Characteristics tissue: spleen
treatment: intact spleen
Treatment protocol spleen samples were cellected on different time periods after 70% liver resection/without resection (intact)
Growth protocol Spleen samples were collected, frozen, lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from intact spleen
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
Submission date May 15, 2023
Last update date Sep 27, 2023
Contact name Polina Vishnyakova
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
Platform ID GPL23038
Series (1)
GSE232531 Gene expression profile, population of macrophages and lymphocytes of the spleen in conditions of liver regeneration after 70% resection in mice

Data table header descriptions
VALUE log2 signal

Data table
AFFX-BkGr-GC03_st 4.62882 0.74407
AFFX-BkGr-GC04_st 5.35575 0.908995
AFFX-BkGr-GC05_st 5.34382 0.967172
AFFX-BkGr-GC06_st 5.01981 0.978755
AFFX-BkGr-GC07_st 4.42193 0.974098
AFFX-BkGr-GC08_st 3.61443 0.976017
AFFX-BkGr-GC09_st 3.25617 0.962946
AFFX-BkGr-GC10_st 3.12317 0.952544
AFFX-BkGr-GC11_st 2.9571 0.926934
AFFX-BkGr-GC12_st 2.7427 0.877165
AFFX-BkGr-GC13_st 2.55218 0.917395
AFFX-BkGr-GC14_st 2.36953 0.902544
AFFX-BkGr-GC15_st 2.39617 0.901118
AFFX-BkGr-GC16_st 2.60606 0.830141
AFFX-BkGr-GC17_st 2.57042 0.809983
AFFX-BkGr-GC18_st 3.01472 0.742941
AFFX-BkGr-GC19_st 4.81355 0.644769
AFFX-BkGr-GC20_st 4.92187 0.630509
AFFX-BkGr-GC21_st 5.00351 0.625433
AFFX-BkGr-GC22_st 5.15217 0.620892

Total number of rows: 28846

Table truncated, full table size 1051 Kbytes.

Supplementary file Size Download File type/resource
GSM7349921_Mus_6_22_Clariom_S_Mouse.CEL.gz 1015.9 Kb (ftp)(http) CEL
GSM7349921_Mus_6_22_Clariom_S_Mouse.sst-rma-gene-full.chp.gz 320.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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