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Sample GSM7349924 Query DataSets for GSM7349924
Status Public on Sep 27, 2023
Title 1 day resected spleen, biological rep 3
Sample type RNA
Source name spleen RNA
Organism Mus musculus
Characteristics tissue: spleen
treatment: spleen 1 days after resection
Treatment protocol spleen samples were cellected on different time periods after 70% liver resection/without resection (intact)
Growth protocol Spleen samples were collected, frozen, lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip™ Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from spleen 1 days after resection
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
Submission date May 15, 2023
Last update date Sep 27, 2023
Contact name Polina Vishnyakova
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
Platform ID GPL23038
Series (1)
GSE232531 Gene expression profile, population of macrophages and lymphocytes of the spleen in conditions of liver regeneration after 70% resection in mice

Data table header descriptions
VALUE log2 signal

Data table
AFFX-BkGr-GC03_st 5.84962 0.723093
AFFX-BkGr-GC04_st 5.41554 0.925514
AFFX-BkGr-GC05_st 5.34382 0.968696
AFFX-BkGr-GC06_st 5.01981 0.977627
AFFX-BkGr-GC07_st 4.43355 0.973535
AFFX-BkGr-GC08_st 3.65376 0.977031
AFFX-BkGr-GC09_st 3.31482 0.959011
AFFX-BkGr-GC10_st 3.12317 0.956959
AFFX-BkGr-GC11_st 2.9571 0.941736
AFFX-BkGr-GC12_st 2.76217 0.896574
AFFX-BkGr-GC13_st 2.56174 0.929444
AFFX-BkGr-GC14_st 2.44861 0.930105
AFFX-BkGr-GC15_st 2.423 0.896213
AFFX-BkGr-GC16_st 2.60659 0.855912
AFFX-BkGr-GC17_st 2.61381 0.840503
AFFX-BkGr-GC18_st 3.03479 0.766089
AFFX-BkGr-GC19_st 4.81355 0.653262
AFFX-BkGr-GC20_st 4.92187 0.647873
AFFX-BkGr-GC21_st 5.00351 0.640309
AFFX-BkGr-GC22_st 5.15217 0.631987

Total number of rows: 28846

Table truncated, full table size 1049 Kbytes.

Supplementary file Size Download File type/resource
GSM7349924_Mus_10_22_Clariom_S_Mouse.CEL.gz 989.2 Kb (ftp)(http) CEL
GSM7349924_Mus_10_22_Clariom_S_Mouse.sst-rma-gene-full.chp.gz 320.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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