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Sample GSM736983 Query DataSets for GSM736983
Status Public on Jun 30, 2011
Title INS-GAS male mice with H. pylori infection treated with Tamoxifen 1
Sample type RNA
 
Source name INS-GAS mouse stomach, Helicobacter pylori, Tamoxifen
Organism Mus musculus
Characteristics tissue: stomach
gender: male
infectious agent: Helicobacter pylori
duration of infection: 28 weeks
hormone treatment: Tamoxifen
treatment duration: 12 weeks
transgene: INS-GAS
genetic background: FVB/N
Treatment protocol Infected with H pylori for 28 weeks. Treated with subcutaneous pellet of 0.25mg 17beta-estradiol, 15mg Tamoxfien or 5mg placebo for 12 weeks
Growth protocol Mice maintained under normal husbandry conditions
Extracted molecule total RNA
Extraction protocol Trizol and purified with RNeasy/DNase kit
Label Cy3
Label protocol Biotinylated cRNA were prepared according to the standard One-Color Microarray-Based gene expression Analysis, low input quick amp labeling protocol (AGILENT) protocol
 
Hybridization protocol Following fragmentation, cRNA was hybridized on Agilent 4X44K Mouse genome array (Part #G4122F. Design #014868)
Scan protocol GeneChips were scanned using Agilent Microarray Scanner for one color setting for the 44K array
Description Gene expression data from INS-GAS mouse stomachs chronically infected with H. pylori and treated with Tamoxifen
Data processing Data were quantile normalized and read with Feature Extraction software 9.1
 
Submission date Jun 03, 2011
Last update date Jun 30, 2011
Contact name Alexander Sheh
E-mail(s) alexsheh@mit.edu
Organization name MIT
Department Biological Engineering
Lab Fox
Street address 77 Massachusetts ave 16-828
City Cambridge
State/province ma
ZIP/Postal code 02139
Country USA
 
Platform ID GPL7202
Series (1)
GSE29715 mRNA expression data from mice infected with H. pylori treated with estradiol, tamoxifen, or placebo

Data table header descriptions
ID_REF
VALUE gProcessedSignal

Data table
ID_REF VALUE
A_51_P100021 133.862
A_51_P100034 4187.5
A_51_P100052 79.3087
A_51_P100063 3390.97
A_51_P100084 116.2
A_51_P100099 524.479
A_51_P100155 3268.81
A_51_P100174 208.621
A_51_P100181 434.458
A_51_P100218 76.2992
A_51_P100227 1729.01
A_51_P100238 129.827
A_51_P100246 3648.71
A_51_P100289 1835.78
A_51_P100298 628.042
A_51_P100309 66.0464
A_51_P100327 1890.1
A_51_P100347 92.1968
A_51_P100379 118.712
A_51_P100428 65.3369

Total number of rows: 41174

Table truncated, full table size 836 Kbytes.




Supplementary file Size Download File type/resource
GSM736983_Tamoxifen_1.txt.gz 7.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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