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Status |
Public on Jun 30, 2011 |
Title |
INS-GAS male mice with H. pylori infection treated with Tamoxifen 1 |
Sample type |
RNA |
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Source name |
INS-GAS mouse stomach, Helicobacter pylori, Tamoxifen
|
Organism |
Mus musculus |
Characteristics |
tissue: stomach gender: male infectious agent: Helicobacter pylori duration of infection: 28 weeks hormone treatment: Tamoxifen treatment duration: 12 weeks transgene: INS-GAS genetic background: FVB/N
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Treatment protocol |
Infected with H pylori for 28 weeks. Treated with subcutaneous pellet of 0.25mg 17beta-estradiol, 15mg Tamoxfien or 5mg placebo for 12 weeks
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Growth protocol |
Mice maintained under normal husbandry conditions
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol and purified with RNeasy/DNase kit
|
Label |
Cy3
|
Label protocol |
Biotinylated cRNA were prepared according to the standard One-Color Microarray-Based gene expression Analysis, low input quick amp labeling protocol (AGILENT) protocol
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|
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Hybridization protocol |
Following fragmentation, cRNA was hybridized on Agilent 4X44K Mouse genome array (Part #G4122F. Design #014868)
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Scan protocol |
GeneChips were scanned using Agilent Microarray Scanner for one color setting for the 44K array
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Description |
Gene expression data from INS-GAS mouse stomachs chronically infected with H. pylori and treated with Tamoxifen
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Data processing |
Data were quantile normalized and read with Feature Extraction software 9.1
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|
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Submission date |
Jun 03, 2011 |
Last update date |
Jun 30, 2011 |
Contact name |
Alexander Sheh |
E-mail(s) |
alexsheh@mit.edu
|
Organization name |
MIT
|
Department |
Biological Engineering
|
Lab |
Fox
|
Street address |
77 Massachusetts ave 16-828
|
City |
Cambridge |
State/province |
ma |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE29715 |
mRNA expression data from mice infected with H. pylori treated with estradiol, tamoxifen, or placebo |
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