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Sample GSM7371876 Query DataSets for GSM7371876
Status Public on May 18, 2024
Title Normal liver tissues, wild-type C57BL/6 mice, male, 9-month
Sample type SRA
 
Source name Normal liver
Organism Mus musculus
Characteristics tissue: Normal liver
genotype: WT
Sex: Male
strain: C57BL/6
age: 9 months
treatment: none
Extracted molecule total RNA
Extraction protocol RNA was harvested using mir VanaTM miRNA Isolation kit (Cat# AM1561, Ambion). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using VAHTS Stranded mRNA-seq Library Prep Kit following manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description MW
Data processing fastx (version:0.0.13)
Sequence reads were trimmed for adaptor sequence/low-quality sequence using fastx (version:0.0.13) (parameter- Quality limit: 0.01).
Trimmed sequence reads were mapped to GrCM38/mm10 using tophat (version:2.0.9) (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8).
Read count extraction and normalization were performed using cufflink (version:2.1.1).
Differential genes analysis was performed using cuffdiff.
Assembly: mm10
Supplementary files format and content: gene_expression.txt: Tab-delimited text file includes count and FPKM values for each Sample.
 
Submission date May 17, 2023
Last update date May 18, 2024
Contact name aiguo Wang
Organization name Dalian Medical University
Street address DaLian
City DaLian
ZIP/Postal code 116044
Country China
 
Platform ID GPL17021
Series (1)
GSE232694 Transcriptomic Landscape of Hras12V Oncogene-Induced Hepatocarcinogenesis with Gender Disparity
Relations
BioSample SAMN35130997
SRA SRX20414898

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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