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Sample GSM7372057

Query DataSets for GSM7372057

Status

Public on Jun 30, 2023

Title

Plasma, baseline, resting, Male 2

Sample type

SRA

Source name

Blood Plasma

Organism

Homo sapiens

Characteristics

tissue: Blood Plasma
Sex: Male
treatment: None

Extracted molecule

total RNA

Extraction protocol

RNA extraction using Norgen Biotek's Plasma/Serum Exosome Purification and RNA isolation Mini Kit, according to manufacturer's instructions
Single-end libraries constructed from 5 ng of EV RNA using QIAseq miRNA library kits from Qiagen according to manufacturer's recommendations for low yield sampels.

Library strategy

miRNA-Seq

Library source

transcriptomic

Library selection

size fractionation

Instrument model

Illumina NextSeq 500

Description

M56-TP1-Pre

Data processing

Quality control completed with FASTQC from Illumina (Version 0.11.5), no trimming required
Reads were mapped to the designated reference genome (miRbase_v22) using the Qiagen GeneGlobe RNA-seq analysis portal (Version 3.0.1) with default parameters for miRNA analysis
Differential gene expression analysis was performed using the edgeR workflow
Assembly: miRbase_v22
Supplementary files format and content: Excel spreadsheet with multiple tabs that include comparisons of data and the raw counts and CPM for each sample as well as differential expression data for each comparison

Submission date

May 17, 2023

Last update date

Jun 30, 2023

Contact name

Brad Nindl

E-mail(s)

bnindl@pitt.edu

Organization name

University of Pittsburgh

Street address

3860 South Water