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Status |
Public on Jun 30, 2023 |
Title |
Plasma, baseline, post acute, Male 5 |
Sample type |
SRA |
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Source name |
Blood Plasma
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Organism |
Homo sapiens |
Characteristics |
tissue: Blood Plasma Sex: Male treatment: Acute Exercise
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Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction using Norgen Biotek's Plasma/Serum Exosome Purification and RNA isolation Mini Kit, according to manufacturer's instructions Single-end libraries constructed from 5 ng of EV RNA using QIAseq miRNA library kits from Qiagen according to manufacturer's recommendations for low yield sampels.
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Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina NextSeq 500 |
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Description |
M65-TP1-PWO
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Data processing |
Quality control completed with FASTQC from Illumina (Version 0.11.5), no trimming required Reads were mapped to the designated reference genome (miRbase_v22) using the Qiagen GeneGlobe RNA-seq analysis portal (Version 3.0.1) with default parameters for miRNA analysis Differential gene expression analysis was performed using the edgeR workflow Assembly: miRbase_v22 Supplementary files format and content: Excel spreadsheet with multiple tabs that include comparisons of data and the raw counts and CPM for each sample as well as differential expression data for each comparison
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Submission date |
May 17, 2023 |
Last update date |
Jun 30, 2023 |
Contact name |
Brad Nindl |
E-mail(s) |
bnindl@pitt.edu
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Organization name |
University of Pittsburgh
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Street address |
3860 South Water
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City |
Pittsburgh |
ZIP/Postal code |
15203 |
Country |
USA |
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Platform ID |
GPL18573 |
Series (1) |
GSE232700 |
Extracellular Vesicle miRNA response to resistance exercise training in men and women |
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Relations |
BioSample |
SAMN35132087 |
SRA |
SRX20412641 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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