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Sample GSM7372070 Query DataSets for GSM7372070
Status Public on Jun 30, 2023
Title Plasma, baseline, post acute, Male 5
Sample type SRA
 
Source name Blood Plasma
Organism Homo sapiens
Characteristics tissue: Blood Plasma
Sex: Male
treatment: Acute Exercise
Extracted molecule total RNA
Extraction protocol RNA extraction using Norgen Biotek's Plasma/Serum Exosome Purification and RNA isolation Mini Kit, according to manufacturer's instructions
Single-end libraries constructed from 5 ng of EV RNA using QIAseq miRNA library kits from Qiagen according to manufacturer's recommendations for low yield sampels.
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina NextSeq 500
 
Description M65-TP1-PWO
Data processing Quality control completed with FASTQC from Illumina (Version 0.11.5), no trimming required
Reads were mapped to the designated reference genome (miRbase_v22) using the Qiagen GeneGlobe RNA-seq analysis portal (Version 3.0.1) with default parameters for miRNA analysis
Differential gene expression analysis was performed using the edgeR workflow
Assembly: miRbase_v22
Supplementary files format and content: Excel spreadsheet with multiple tabs that include comparisons of data and the raw counts and CPM for each sample as well as differential expression data for each comparison
 
Submission date May 17, 2023
Last update date Jun 30, 2023
Contact name Brad Nindl
E-mail(s) bnindl@pitt.edu
Organization name University of Pittsburgh
Street address 3860 South Water
City Pittsburgh
ZIP/Postal code 15203
Country USA
 
Platform ID GPL18573
Series (1)
GSE232700 Extracellular Vesicle miRNA response to resistance exercise training in men and women
Relations
BioSample SAMN35132087
SRA SRX20412641

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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