|
| Status |
Public on Sep 01, 2011 |
| Title |
NSPCs grown in EC/NSPC co-cultures (microRNA) rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Neural stem/progenitor cells isolated from E14 mouse embryonic brain, grown in co-culture with mouse primary brain Endothelial cells (Celprogen)
|
| Organism |
Mus musculus |
| Characteristics |
sample type: ECs isolated from the mouse forebrain microvessels and NSPCs from telencephalon of E-14 mouse embryo
cell type: Primary neural stem/progenitor cells
treatment: grown in co-culture with mouse primary brain Endothelial cells (Celprogen)
genetic background: C57BL/6
|
| Treatment protocol |
All experimantal samples were grown in growth factor-free NSPC medium (with addition of 10% FBS), for 24 hours, in CO2 incubator at 37C
|
| Growth protocol |
For cell growth and propagation: NSPCs were grown in serum-free NSPC medium and ECs were grown in EC medium (Celprogen), at 37C, in CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24 hours in transwell co-culture, the ECs and NSPCs were separated and miRNA were isolated using mirVana miRNA (AB/Ambion) isolation kit. FlashPAGE fractionator (AB/Ambion) was used for rapid PAGE-purification of miRNAs.
|
| Label |
biotin
|
| Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip miRNA Procedure (FluidicsScript 450-0003, Hyb. 48°C)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
miRNA expression data from NSPCs grown in EC/NSPC co-cultures
|
| Data processing |
Data files were analyzed with Affymetrix miRNAQCtool using RMA background correction and quartile normalization (signal data as log2)
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Tamara Roitbak |
| E-mail(s) |
troitbak@salud.unm.edu, ggpickett@salud.unm.edu
|
| Phone |
505 272-3401
|
| Fax |
505 272-6091
|
| Organization name |
UNM
|
| Department |
Neurosurgery
|
| Street address |
2212 Lomas NE
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE29759 |
The Role of microRNAs in Neural Stem Cell-supported Endothelial Morphogenesis |
|
