|
| Status |
Public on Sep 01, 2011 |
| Title |
ECs grown in EC/NSPC co-cultures (mRNA) rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse primary brain endothelial cells (Celprogen) grown in co-culture with neural stem/progenitor cells isolated from E14 mouse embryonic brain
|
| Organism |
Mus musculus |
| Characteristics |
sample type: ECs isolated from the mouse forebrain microvessels and NSPCs from telencephalon of E-14 mouse embryo
cell type: Primary brain endothelial cells (Celprogen, San Diego, CA, catalog # 11053-07)
treatment: grown in co-culture with neural stem/progenitor cells isolated from E14 mouse embryonic brain
|
| Treatment protocol |
All experimantal samples were grown in growth factor-free NSPC medium (with addition of 10% FBS), for 24 hours, in CO2 incubator at 37C
|
| Growth protocol |
For cell growth and propagation: NSPCs were grown in serum-free NSPC medium and ECs were grown in EC medium (Celprogen), at 37C, in CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24 hours in transwell co-culture, the ECs and NSPCs were separated and total RNA was isolated using RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Affymetrix 3' IVT Express labeling kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
RNA expression data from ECs grown in EC/NSPC co-cultures
|
| Data processing |
The data were analyzed with RMA and Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Tamara Roitbak |
| E-mail(s) |
troitbak@salud.unm.edu, ggpickett@salud.unm.edu
|
| Phone |
505 272-3401
|
| Fax |
505 272-6091
|
| Organization name |
UNM
|
| Department |
Neurosurgery
|
| Street address |
2212 Lomas NE
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE29759 |
The Role of microRNAs in Neural Stem Cell-supported Endothelial Morphogenesis |
|
