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Sample GSM740114 Query DataSets for GSM740114
Status Public on Nov 06, 2012
Title Stump_rep3 [Exiqon]
Sample type RNA
 
Channel 1
Source name Non-regenerating stump
Organism Ambystoma mexicanum
Characteristics days post amputation: 0
tissue: Non-regenerating stump
Extracted molecule total RNA
Extraction protocol Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
Label Hy3
Label protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
 
Channel 2
Source name Common Reference
Organism Ambystoma mexicanum
Characteristics sample type: Common Reference (made by pooling all the samples)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
Label Hy5
Label protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
 
 
Hybridization protocol The samples were hybridized on the miRCURY™ LNA Array (v.9.2) as per Exiqons protocols.
Scan protocol Arrays were scanned as per Exiqons protocols.
Description 378st
Biological Replicate 3 of 3
Slide6
Cy3: 1_Exiqon_13768593_S01_Cropped.txt
Cy5: 0_Exiqon_13768593_S01_Cropped.txt
Data processing Log ratios between the Hy3/Hy5 have been provided along with the normalized independent Hy3 and Hy5 signals. The quantified signals (background correction) were normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
 
Submission date Jun 10, 2011
Last update date Nov 06, 2012
Contact name Edna C Holman
E-mail(s) edna96@hotmail.com
Phone 203-432-6553
URL http://www.yale.edu/crews/
Organization name Yale University
Department Molecular, Cellular and Developmental Biology
Lab Craig Crews
Street address 266 Whitney Ave. KBT-442
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
 
Platform ID GPL7724
Series (1)
GSE29727 Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration

Data table header descriptions
ID_REF
VALUE LOWESS normalized log2(Hy3/Hy5) ratios, meaning sample/pool

Data table
ID_REF VALUE
17915 -0.39
17521 0.20
5740 -1.71
17739 0.53
17904 -0.27
17322 -0.06
17400 -1.27
11048 0.23
17871 -0.56
21472 -0.51
17695 -0.75
14458 -1.05
25324 0.42
10935 0.20
19581 0.25
28431 -0.86
11264 -0.26
13148 0.31
17939 -0.52
11057 0.10

Total number of rows: 2028

Table truncated, full table size 15 Kbytes.




Supplementary file Size Download File type/resource
GSM740114_0_Exiqon_13768593_S01_Cropped.txt.gz 914.6 Kb (ftp)(http) TXT
GSM740114_1_Exiqon_13768593_S01_Cropped.txt.gz 1006.8 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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