|
Status |
Public on Nov 06, 2012 |
Title |
Stump_rep3 [Exiqon] |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Non-regenerating stump
|
Organism |
Ambystoma mexicanum |
Characteristics |
days post amputation: 0 tissue: Non-regenerating stump
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
Label |
Hy3
|
Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
|
|
|
Channel 2 |
Source name |
Common Reference
|
Organism |
Ambystoma mexicanum |
Characteristics |
sample type: Common Reference (made by pooling all the samples)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
Label |
Hy5
|
Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
|
|
|
|
Hybridization protocol |
The samples were hybridized on the miRCURY™ LNA Array (v.9.2) as per Exiqons protocols.
|
Scan protocol |
Arrays were scanned as per Exiqons protocols.
|
Description |
378st Biological Replicate 3 of 3 Slide6 Cy3: 1_Exiqon_13768593_S01_Cropped.txt Cy5: 0_Exiqon_13768593_S01_Cropped.txt
|
Data processing |
Log ratios between the Hy3/Hy5 have been provided along with the normalized independent Hy3 and Hy5 signals. The quantified signals (background correction) were normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
|
|
Submission date |
Jun 10, 2011 |
Last update date |
Nov 06, 2012 |
Contact name |
Edna C Holman |
E-mail(s) |
edna96@hotmail.com
|
Phone |
203-432-6553
|
URL |
http://www.yale.edu/crews/
|
Organization name |
Yale University
|
Department |
Molecular, Cellular and Developmental Biology
|
Lab |
Craig Crews
|
Street address |
266 Whitney Ave. KBT-442
|
City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06511 |
Country |
USA |
|
|
Platform ID |
GPL7724 |
Series (1) |
GSE29727 |
Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration |
|