|
| Status |
Public on Nov 22, 2011 |
| Title |
Old_utx-1_H3K27me3_ChIPSeq |
| Sample type |
SRA |
| |
|
| Source name |
Whole worm, old, Utx-1 RNAi, H3K27me3 ChIP
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
tissue: whole body
age: 10 days
treatment: Utx-1 RNAi
chip antibody: H3K27me3
|
| Treatment protocol |
Synchronized eggs grown to young adult stage were distributed to RNAi or non-RNAi plates containing 20 ug/ml FUdR to prevent progeny growth. For RNAi, bacteria clones were selected from Ahringer's RNAi feeding bacteria library (Kamath et al, 2003), and dsRNA expression was induced by 1mM IPTG. The same HT115 bacteria carrying the empty L4440 construct were used as controls in all experiments. The worms were cultured at 20oC and transferred to fresh RNAi plates every 4 days to ensure continued efficacy of RNAi knockdown.
|
| Growth protocol |
C. elegans were cultured on plates with 10x condensed food. Young and Old samples were collected on adult days 3 and 10, respectively.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seq library construction, cluster generation and sequencing analysis using the Illumina 1G Genome Analyzer were performed as described by the manufacturer's protocols.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Old Utx-1 RNAi treated
|
| Data processing |
Alignment: Raw data were mapped to the ce6 genome by Soap2.
|
| |
|
| Submission date |
Jun 10, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Dali Han |
| E-mail(s) |
handali294@gmail.com
|
| Organization name |
University of Chicago
|
| Department |
Department of Chemisty
|
| Street address |
5801 South Ellis Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL9309 |
| Series (1) |
| GSE29896 |
Histone Demethylase UTX-1 Regulates the Caenorhabditis elegans Lifespan by Targeting the Insulin/IGF-1 Signaling Pathway |
|
| Relations |
| SRA |
SRX076079 |
| BioSample |
SAMN00627021 |
| Named Annotation |
GSM740294.bed.gz |
