|
| Status |
Public on Jun 13, 2011 |
| Title |
D17C0A_ |
| Sample type |
RNA |
| |
|
| Source name |
HAEC treated with 40ug/ml PAPC
|
| Organism |
Homo sapiens |
| Characteristics |
individual identifier: 17
cell line: human aortic endothelial cell (HAEC) cell line
agent: PAPC (control)
dose: 40ug/ml
|
| Treatment protocol |
Cells were treated with PAPC or oxPAPC (10–50 μg/ml), in medium containing 1% FBS.
|
| Growth protocol |
Human aortic endothelial cells were isolated from aortic samples retrieved at the time of organ harvest for cardiac transplantation and used at passages 4–7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Double stranded cDNA were prepared with the Invitrogen reverse transcription kit. Biotin-labeled cRNA was generated and used to probe Affymetrix arrays
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
Gene expression data from HAEC
31388
Supplementary file .TXT represents Expression Analysis: Metrics Tab.
|
| Data processing |
The data were normalised using median invariant normalization with Microarray Suite 5.0 software.
|
| |
|
| Submission date |
Jun 12, 2011 |
| Last update date |
Jun 13, 2011 |
| Contact name |
Juyong Brian Kim |
| E-mail(s) |
jkim@mednet.ucla.edu
|
| Phone |
310-825-1595
|
| Fax |
310-794-7345
|
| Organization name |
UCLA School of Medicine
|
| Department |
Department of Medicine
|
| Lab |
Lusis Lab
|
| Street address |
675 Charles Young Dr.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29903 |
Identification of inflammatory gene modules based on variations of human endothelial cell responses to oxidized lipids |
|
