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Sample GSM743806 Query DataSets for GSM743806
Status Public on May 22, 2012
Title Biotin-HSR labeled brain samples, 1000ng total RNA, technical rep2
Sample type RNA
 
Source name Brain, 1000ng RNA, biotin-HSR label
Organism Homo sapiens
Characteristics tissue: brain
rna amount: 1000ng
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
Label biotin-HSR
Label protocol The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website (www.genisphere.com) or by contacting the kit manufacturer.
 
Hybridization protocol The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at www.genisphere.com or www.affymetrix.com.
Scan protocol Standard Affymetrix protocol.
Description miRNA-1_0_2Xgain library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
 
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
E-mail(s) jkaddis@coh.org
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
 
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
ID_REF
VALUE Processed signal intensity values, log2 transformed

Data table
ID_REF VALUE
AFFX-BioB-5_at 11.44853
AFFX-BioB-M_at 11.7146
AFFX-BioB-3_at 11.66459
AFFX-BioC-5_at 12.06058
AFFX-BioC-3_at 11.69242
AFFX-BioDn-5_at 12.47002
AFFX-BioDn-3_at 12.90271
AFFX-CreX-5_at 13.58002
AFFX-CreX-3_at 13.66242
AFFX-r2-Ec-c1-bioB-5_at 11.44413
AFFX-r2-Ec-c1-bioB-M_at 11.69504
AFFX-r2-Ec-c1-bioB-3_at 11.36336
AFFX-r2-Ec-c1-bioC-5_at 12.365
AFFX-r2-Ec-c1-bioC-3_at 12.28883
AFFX-r2-Ec-c1-bioD-5_at 12.61667
AFFX-r2-Ec-c1-bioD-3_at 12.83323
AFFX-r2-P1-c1-cre-5_at 13.76554
AFFX-r2-P1-c1-cre-3_at 13.91043
AFFX-r2-Ec-c2-bioB-5_at 11.48957
AFFX-r2-Ec-c2-bioB-M_at 11.74068

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.




Supplementary file Size Download File type/resource
GSM743806.CEL.gz 179.7 Kb (ftp)(http) CEL
Processed data included within Sample table

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