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Sample GSM743808 Query DataSets for GSM743808
Status Public on May 22, 2012
Title Biotin-HSR labeled lung samples, 100ng total RNA, technical rep2
Sample type RNA
Source name Lung, 100ng RNA, biotin-HSR label
Organism Homo sapiens
Characteristics tissue: lung
rna amount: 100ng
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
Label biotin-HSR
Label protocol The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website ( or by contacting the kit manufacturer.
Hybridization protocol The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at or
Scan protocol Standard Affymetrix protocol.
Description miRNA-1_0_2Xgain library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
VALUE Processed signal intensity values, log2 transformed

Data table
AFFX-BioB-5_at 12.03308
AFFX-BioB-M_at 12.22921
AFFX-BioB-3_at 12.29807
AFFX-BioC-5_at 12.6482
AFFX-BioC-3_at 12.22728
AFFX-BioDn-5_at 13.16017
AFFX-BioDn-3_at 13.78823
AFFX-CreX-5_at 14.23679
AFFX-CreX-3_at 14.34645
AFFX-r2-Ec-c1-bioB-5_at 11.92067
AFFX-r2-Ec-c1-bioB-M_at 12.09664
AFFX-r2-Ec-c1-bioB-3_at 11.91154
AFFX-r2-Ec-c1-bioC-5_at 12.96791
AFFX-r2-Ec-c1-bioC-3_at 12.82247
AFFX-r2-Ec-c1-bioD-5_at 13.50097
AFFX-r2-Ec-c1-bioD-3_at 13.6239
AFFX-r2-P1-c1-cre-5_at 14.41084
AFFX-r2-P1-c1-cre-3_at 14.53631
AFFX-r2-Ec-c2-bioB-5_at 11.99961
AFFX-r2-Ec-c2-bioB-M_at 12.07292

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.

Supplementary file Size Download File type/resource
GSM743808.CEL.gz 164.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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