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Sample GSM743809 Query DataSets for GSM743809
Status Public on May 22, 2012
Title Biotin-HSR labeled lung samples, 200ng total RNA, technical rep1
Sample type RNA
Source name Lung, 200ng RNA, biotin-HSR label
Organism Homo sapiens
Characteristics tissue: lung
rna amount: 200ng
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
Label biotin-HSR
Label protocol The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website ( or by contacting the kit manufacturer.
Hybridization protocol The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at or
Scan protocol Standard Affymetrix protocol.
Description miRNA-1_0_2Xgain library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
VALUE Processed signal intensity values, log2 transformed

Data table
AFFX-BioB-5_at 11.66943
AFFX-BioB-M_at 11.9702
AFFX-BioB-3_at 11.94421
AFFX-BioC-5_at 12.35385
AFFX-BioC-3_at 12.00155
AFFX-BioDn-5_at 12.97452
AFFX-BioDn-3_at 13.57718
AFFX-CreX-5_at 14.25859
AFFX-CreX-3_at 14.31248
AFFX-r2-Ec-c1-bioB-5_at 11.66969
AFFX-r2-Ec-c1-bioB-M_at 11.90102
AFFX-r2-Ec-c1-bioB-3_at 11.71736
AFFX-r2-Ec-c1-bioC-5_at 12.69381
AFFX-r2-Ec-c1-bioC-3_at 12.53552
AFFX-r2-Ec-c1-bioD-5_at 13.28416
AFFX-r2-Ec-c1-bioD-3_at 13.4674
AFFX-r2-P1-c1-cre-5_at 14.43617
AFFX-r2-P1-c1-cre-3_at 14.57865
AFFX-r2-Ec-c2-bioB-5_at 11.71708
AFFX-r2-Ec-c2-bioB-M_at 11.89223

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.

Supplementary file Size Download File type/resource
GSM743809.CEL.gz 172.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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