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Sample GSM743818 Query DataSets for GSM743818
Status Public on May 22, 2012
Title Biotin-Only labeled brain samples, 200ng total RNA, technical rep2
Sample type RNA
Source name Brain, 200ng RNA, biotin-only label
Organism Homo sapiens
Characteristics tissue: brain
rna amount: 200ng
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
Label biotin
Label protocol The Genisphere FlashTag Biotin Kit was used. The protocol is available for download at the product website ( or by contacting the kit manufacturer.
Hybridization protocol The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at or
Scan protocol Standard Affymetrix protocol.
Description miRNA-1_0 library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
VALUE Processed signal intensity values, log2 transformed

Data table
AFFX-BioB-5_at 12.64604
AFFX-BioB-M_at 12.7681
AFFX-BioB-3_at 12.84938
AFFX-BioC-5_at 13.08343
AFFX-BioC-3_at 12.74619
AFFX-BioDn-5_at 13.48053
AFFX-BioDn-3_at 13.91926
AFFX-CreX-5_at 14.36032
AFFX-CreX-3_at 14.3772
AFFX-r2-Ec-c1-bioB-5_at 12.53588
AFFX-r2-Ec-c1-bioB-M_at 12.66429
AFFX-r2-Ec-c1-bioB-3_at 12.58863
AFFX-r2-Ec-c1-bioC-5_at 13.29282
AFFX-r2-Ec-c1-bioC-3_at 13.26933
AFFX-r2-Ec-c1-bioD-5_at 13.67745
AFFX-r2-Ec-c1-bioD-3_at 13.76434
AFFX-r2-P1-c1-cre-5_at 14.48764
AFFX-r2-P1-c1-cre-3_at 14.53824
AFFX-r2-Ec-c2-bioB-5_at 12.59781
AFFX-r2-Ec-c2-bioB-M_at 12.7271

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.

Supplementary file Size Download File type/resource
GSM743818.CEL.gz 137.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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