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Sample GSM743829 Query DataSets for GSM743829
Status Public on May 22, 2012
Title Biotin-Only labeled lung samples, 1000ng total RNA, technical rep1
Sample type RNA
Source name Lung, 1000ng RNA, biotin-only label
Organism Homo sapiens
Characteristics tissue: lung
rna amount: 1000ng
Extracted molecule total RNA
Extraction protocol Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
Label biotin
Label protocol The Genisphere FlashTag Biotin Kit was used. The protocol is available for download at the product website ( or by contacting the kit manufacturer.
Hybridization protocol The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at or
Scan protocol Standard Affymetrix protocol.
Description miRNA-1_0 library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
VALUE Processed signal intensity values, log2 transformed

Data table
AFFX-BioB-5_at 11.64083
AFFX-BioB-M_at 11.96549
AFFX-BioB-3_at 11.91753
AFFX-BioC-5_at 12.28955
AFFX-BioC-3_at 11.91972
AFFX-BioDn-5_at 12.84024
AFFX-BioDn-3_at 13.42037
AFFX-CreX-5_at 13.99485
AFFX-CreX-3_at 14.16562
AFFX-r2-Ec-c1-bioB-5_at 11.68398
AFFX-r2-Ec-c1-bioB-M_at 11.89109
AFFX-r2-Ec-c1-bioB-3_at 11.70911
AFFX-r2-Ec-c1-bioC-5_at 12.56121
AFFX-r2-Ec-c1-bioC-3_at 12.48913
AFFX-r2-Ec-c1-bioD-5_at 13.16982
AFFX-r2-Ec-c1-bioD-3_at 13.31143
AFFX-r2-P1-c1-cre-5_at 14.15224
AFFX-r2-P1-c1-cre-3_at 14.4051
AFFX-r2-Ec-c2-bioB-5_at 11.71135
AFFX-r2-Ec-c2-bioB-M_at 11.89211

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.

Supplementary file Size Download File type/resource
GSM743829.CEL.gz 158.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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