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Sample GSM743832 Query DataSets for GSM743832
Status Public on May 22, 2012
Title Biotin-HSR labeled synthetic miRNA spike in, 4.88 attomoles
Sample type other
 
Source name Synthetic oligos, 4.88 attomoles
Organism synthetic construct
Characteristics miRNA spike in amount: 4.88 attomoles
Extracted molecule other
Extraction protocol The synthetic miRNAs were obtained from the Ambion mirVana miRNA Reference Panel v9.1 (Lot number 072307).
Label biotin-HSR
Label protocol The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website (www.genisphere.com) or by contacting the kit manufacturer.
 
Hybridization protocol The quantity of miRNA spike in varied from 5000 to 4.88 attomoles.
Scan protocol Standard Affymetrix protocol.
Description Synthetic human, rat, and mouse miRNAs.
miRNA-1_0_2Xgain library file used for scanning.
Data processing Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
 
Submission date Jun 16, 2011
Last update date May 23, 2012
Contact name John S. Kaddis
E-mail(s) jkaddis@coh.org
Organization name City of Hope
Department Diabetes and Cancer Discovery Science
Street address 1500 East Duarte Road
City Duarte
State/province CA
ZIP/Postal code 91010-3000
Country USA
 
Platform ID GPL8786
Series (1)
GSE30045 Influence of RNA Labeling on Expression Profiling of MicroRNAs

Data table header descriptions
ID_REF
VALUE Processed signal intensity values, log2 transformed

Data table
ID_REF VALUE
AFFX-BioB-5_at 12.37205
AFFX-BioB-M_at 13.02156
AFFX-BioB-3_at 13.06109
AFFX-BioC-5_at 13.34235
AFFX-BioC-3_at 12.81615
AFFX-BioDn-5_at 13.7284
AFFX-BioDn-3_at 14.08499
AFFX-CreX-5_at 14.49708
AFFX-CreX-3_at 14.57096
AFFX-r2-Ec-c1-bioB-5_at 12.25722
AFFX-r2-Ec-c1-bioB-M_at 12.61073
AFFX-r2-Ec-c1-bioB-3_at 12.24255
AFFX-r2-Ec-c1-bioC-5_at 13.69655
AFFX-r2-Ec-c1-bioC-3_at 13.62517
AFFX-r2-Ec-c1-bioD-5_at 13.85089
AFFX-r2-Ec-c1-bioD-3_at 13.96493
AFFX-r2-P1-c1-cre-5_at 14.66321
AFFX-r2-P1-c1-cre-3_at 14.79005
AFFX-r2-Ec-c2-bioB-5_at 12.27856
AFFX-r2-Ec-c2-bioB-M_at 12.85341

Total number of rows: 7788

Table truncated, full table size 188 Kbytes.




Supplementary file Size Download File type/resource
GSM743832.CEL.gz 162.0 Kb (ftp)(http) CEL
Processed data included within Sample table

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