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Sample GSM7439159 Query DataSets for GSM7439159
Status Public on Jun 06, 2023
Title UKK2_hiPSC_Buspirone_Day1_R1
Sample type RNA
 
Source name IMR90 hiPSC cells
Organism Homo sapiens
Characteristics gender: female
state: proliferating state
agent: Buspirone
time point: Day1
Treatment protocol Cells were incubated (5 % CO2, 37 °C) with the test compounds at different concentrations and a DMSO concentration of 0.1 % or 0.5 %.
Growth protocol Cells were differentiated in RPMI 1640 GlutaMAX™ medium (Thermo Fisher Scientific, Germany) plus B-27™ Supplement, minus insulin (Thermo Fisher Scientific, Germany) supplemented with 10 µM Wnt activator small molecule CHIR99021 (R&D Systems, Minneapolis, USA).
Extracted molecule total RNA
Extraction protocol The cells were homogenised with TRIzol lysis reagent (Thermo Fisher Scientific, Germany), and the total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions.
Label biotin
Label protocol The samples were amplified and labelled with biotin using GeneChip 3′ IVT Express Kit per the manufacturer’s instructions (Affymetrix, High Wycombe, UK).
 
Hybridization protocol The samples were purified using magnetic beads and fragmented. 12,5 μg of fragmented RNA samples were hybridized on Affymetrix Human Genome U133 Plus 2.0 arrays (Affymetrix, Santa Clara, CA, USA). The microarray hybridization step was performed in an Affymetrix GeneChip Hybridization Oven-645 for 16 h at 45 °C and 60 rpm. Washing and staining of the hybridized arrays was completed using the GeneChip HWS Kit (Affymetrix, High Wycombe, United Kingdom) and Affymetrix GeneChip Fluidics Station-450.
Scan protocol Stained arrays were scanned with Affymetrix Gene-Chip Scanner-3000-7G and evaluated for quality control with Affymetrix GCOS software
Data processing All analyses were conducted using the statistical software R, version 4.2.2. Pre-processing of the data consists of the three steps background correction, normalization, and summarization, using the frozen robust multi-array average (fRMA) algorithm. This yields expression values for 54675 probe sets. The R-packages affy, frma and hgu133plus2frmavecs were used.
 
Submission date Jun 01, 2023
Last update date Jun 06, 2023
Contact name Agapios Sachinidis
E-mail(s) a.sachinidis@uni-koeln.de
Phone +492214787373
Organization name University of Cologne
Department Institute of Neurophysiology
Street address Robert-Kochstr. 39
City Cologne
State/province NRW
ZIP/Postal code 50931
Country Germany
 
Platform ID GPL570
Series (2)
GSE233924 Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [Toxicants]
GSE233926 Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells

Data table header descriptions
ID_REF
VALUE Log2-transformed expression values

Data table
ID_REF VALUE
1007_s_at 8.856878932
1053_at 8.960369379
117_at 5.353363902
121_at 7.62602641
1255_g_at 4.603326125
1294_at 5.875973914
1316_at 5.879298797
1320_at 4.719396076
1405_i_at 3.578454377
1431_at 3.673128518
1438_at 5.833516826
1487_at 7.546392629
1494_f_at 5.261199976
1552256_a_at 8.149631125
1552257_a_at 7.892106728
1552258_at 3.732695397
1552261_at 4.499792074
1552263_at 7.360627366
1552264_a_at 7.811238338
1552266_at 3.267733717

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM7439159_22.a_6.12.21_Anna_HG-U133_Plus_2_.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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