|
| Status |
Public on May 20, 2024 |
| Title |
Uninfected_HiLung-Airway_3 |
| Sample type |
SRA |
| |
|
| Source name |
iPSC-derived airway cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: iPSC-derived airway cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAamp RNA Blood Mini Kit (Qiagen, Cat# 52304)
RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit (illumina) following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequenced reads were trimmed with fastp (v.0.21.0).
Sequenced reads were mapped to the human, SARS-CoV-2, and BANAL-236 reference genomes using STAR (v.2.6.1c).
Assembly: Human (GRCh38.p13), BANAL-236 (MZ937003.2), and SARS-CoV-2 (NC_045512.2)
Supplementary files format and content: Expression read count matrix (counts.txt)
|
| |
|
| Submission date |
Jun 01, 2023 |
| Last update date |
May 20, 2024 |
| Contact name |
Kei Sato |
| E-mail(s) |
su9ark@gmail.com
|
| Phone |
81364092212
|
| Organization name |
The Institute of Medical Science, The University of Tokyo
|
| Department |
Department of Microbiology and Immunology
|
| Lab |
Division of Systems Virology
|
| Street address |
4-6-1 Shiroganedai
|
| City |
Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1088639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE233943 |
RNA-Seq data from iPSC-derived human airway cells and colon organoids infected with BANAL-20-236, WK-521, and WK-521 dFCS |
|
| Relations |
| BioSample |
SAMN35561223 |
| SRA |
SRX20571459 |
