GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM7454063 Query DataSets for GSM7454063
Status Public on Jun 07, 2023
Title H1_WT_2
Sample type SRA
Source name H1 hESC
Organism Homo sapiens
Characteristics cell line: H1 hESC
genotype: WT
treatment: Non-targeting control siRNA
Growth protocol HEK 293T cells were maintained in DMEM/GlutaMAX + 10% FBS in humidified atmosphere with 5% CO2 at 37C
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using the Macherey-Nagel NucleoSpin Mini Kit
RNA libraries for RNA-seq were prepared by Novogene
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing Mapping to reference was performed with hisat2 (Version 2.0.5) using default parameters
Quantification was performed with featureCounts (Version 1.5.0-p3)
Differential analysis was performed using DESeq2 (Version 1.20.0) and edgeR (Version 0.22.0)
Assembly: GRCm38/mm10
Supplementary files format and content: Tab-delimited text file includes FPKM values for each Sample
Submission date Jun 05, 2023
Last update date Jun 07, 2023
Contact name Michael Rapé
Organization name University of California Berkeley
Department Molecular and Cell Biology
Street address 330F Li Ka Shing Center
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platform ID GPL24676
Series (1)
GSE234200 Orphan quality control shapes network dynamics and gene expression
BioSample SAMN35639518
SRA SRX20599674

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap