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Sample GSM7465441 Query DataSets for GSM7465441
Status Public on Jun 09, 2023
Title SP-39c
Sample type SRA
 
Source name CAR-2CCT-T
Organism Homo sapiens
Characteristics treatment: Spleen
cell type: CAR-2CCT-T
Extracted molecule total RNA
Extraction protocol CAR-T cells were sorted using Aria II cell sorter (BD) based on mScarlet expression.
Sorted cells were washed with PBS, and cell number and viability were assessed by trypan blue (Lonza) staining. About 2,000 to 10,000 purified mScarlet+ cells were used for scRNA-seq library preparation using Chromium Next GEM Single Cell 5’ Reagent Kits V2 (10x Genomics) according to manufacturer’s instructions. The single cell libraries were sequenced by NovaSeq 6000 (Illumina) with 2x150 read length.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing raw FASTQ data were pre-processed with Cell Ranger v6.0.1.
Assembly: Ensembl GRCh38
Supplementary files format and content: filtered dataset using Cell Ranger v6.0.1
 
Submission date Jun 07, 2023
Last update date Jun 09, 2023
Contact name Sidi Chen
E-mail(s) sidi.chen@yale.edu
Organization name Yale University
Street address 850 West Campus Drive
City West Haven
State/province CT
ZIP/Postal code 06516
Country USA
 
Platform ID GPL24676
Series (2)
GSE234307 CTLA-4 tail fusion enhances CAR-T anti-tumor immunity
GSE234347 CTLA-4 tail fusion enhances CAR-T anti-tumor immunity [single-cell RNA-seq]
Relations
BioSample SAMN35661608
SRA SRX20624214

Supplementary file Size Download File type/resource
GSM7465441_SP-39c_filtered.h5 16.8 Mb (ftp)(http) H5
GSM7465441_SP-39c_raw.h5 25.7 Mb (ftp)(http) H5
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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