|
Status |
Public on Jun 09, 2023 |
Title |
BM-39a |
Sample type |
SRA |
|
|
Source name |
CAR-T
|
Organism |
Homo sapiens |
Characteristics |
treatment: Bone Marrow cell type: CAR-T
|
Extracted molecule |
total RNA |
Extraction protocol |
CAR-T cells were sorted using Aria II cell sorter (BD) based on mScarlet expression. Sorted cells were washed with PBS, and cell number and viability were assessed by trypan blue (Lonza) staining. About 2,000 to 10,000 purified mScarlet+ cells were used for scRNA-seq library preparation using Chromium Next GEM Single Cell 5’ Reagent Kits V2 (10x Genomics) according to manufacturer’s instructions. The single cell libraries were sequenced by NovaSeq 6000 (Illumina) with 2x150 read length.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
raw FASTQ data were pre-processed with Cell Ranger v6.0.1. Assembly: Ensembl GRCh38 Supplementary files format and content: filtered dataset using Cell Ranger v6.0.1
|
|
|
Submission date |
Jun 07, 2023 |
Last update date |
Jun 09, 2023 |
Contact name |
Sidi Chen |
E-mail(s) |
sidi.chen@yale.edu
|
Organization name |
Yale University
|
Street address |
850 West Campus Drive
|
City |
West Haven |
State/province |
CT |
ZIP/Postal code |
06516 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE234307 |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity |
GSE234347 |
CTLA-4 tail fusion enhances CAR-T anti-tumor immunity [single-cell RNA-seq] |
|
Relations |
BioSample |
SAMN35661606 |
SRA |
SRX20624216 |