|
| Status |
Public on Nov 02, 2012 |
| Title |
COLO320 DNA copy number |
| Sample type |
genomic |
| |
|
| Source name |
COLO320 genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: COLO320
|
| Growth protocol |
COLO320 cells were grown in RPMI-1640 + 10% FBS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from cells using the Qiagen Genomic DNA Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
CEL files were processed by Affymetrix Genotyping Console and copy number analysis performed using the Birdseed algorithm
log2 copy number ratio
|
| |
|
| Submission date |
Jun 23, 2011 |
| Last update date |
Nov 02, 2012 |
| Contact name |
Keyan Salari |
| E-mail(s) |
ksalari@mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Street address |
Gray/Bigelow Rm 1102, 55 Fruit Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL6801 |
| Series (2) |
|
